HPLC Lab
Chromatographic Absolute Precision

HPLC
Analysis

High-Performance Liquid Chromatography services utilizing advanced UV-Vis, PDA, and Fluorescence detectors for unmatched molecular quantitation.

Quantifying Ingredient Integrity

Allele Life Sciences provides industry-leading HPLC analytical services. Equipped with advanced Agilent systems featuring multiple detection capabilities (UV-Vis, PDA, Fluorescence), we ensure the highest sensitivity and accuracy for your molecular separation and quantification requirements.

PDA / UV-Vis detection

Wide absorbance spectrum for organic compounds.

Deformulation Master

Identify impurities and quantify ingredient amounts.

Method Stability

We develop methods focused on Accuracy, Precision, Limit of Detection (LOD), System Suitability, and Linearity, ensuring robust structural degradation and profiling capabilities.

Proteins, Peptides & Antibody Biomolecules
Dyes, Surfactants & Industrial Chemicals
Drug & Intermediate deformulation
High-Sensitivity Fluorometric Arrays

Chromatographic Services

01

Method Development

Validation procedures following accuracy, specificity, Limit of Detection (LOD), Linearity, and robust system stability.

LOD/LOQ Determination
Accuracy & Reproducibility
Sample Suitability checks
02

Biomolecule Quantitation

High-sensitivity quantitation of Proteins, Peptides, and Antibodies using advanced HPLC-PDA/FLD systems.

Fluorescent modification
PDA detection
Protein/Peptide isolation
03

Deformulation Analysis

Comprehensive ingredient reports revealing a product's composition, identifying impurities, and determining CAS numbers.

Raw ingredient quantification
Impurity screening
Formula cost assessment
04

Industrial Chemicals

Analytical separation and characterization of industrial organic compounds, dyes, surfactants, and intermediates.

Drug intermediate testing
Fatty acid profiling
Formaldehyde & allergenic analysis
05

Fluorescence Detection

Highly sensitive fluorometric detection suited for inherently fluorescent compounds or tagged biomolecules.

Laser-induced emission
High signal-to-noise
Trace-level detection
06

Impurity Profiling

Identifying and quantifying potential contaminants in pharmaceuticals and chemicals to ensure regulatory compliance.

Trace impurity limits
Regulatory compliance data
Structural degradation analysis

The Analytical Method

1

Sample Prep

Filtration & formulation

2

Injection

Autosampler insertion

3

Separation

Stationary phase column

4

Detection

UV-Vis, PDA, or FLD

5

Quantitation

Chromatogram analysis

Industrial & Formulative Reverse-EngineeringChemical Intelligence

"Our deformulation reports can reveal a product's complete ingredients. Our testing services can identify potential impurities, determine CAS numbers, quantify the amounts of each raw component, and synthesize a deep analytical cost analysis profile."

Validated Lab Instruments

Pioneering liquid state molecular detection networks.

Agilent Systems

Industry-leading HPLC

UV-Vis/PDA

Spectral absorbance

FLD Optics

Fluorescent emission

C18 Columns

Reverse-phase analytics

Trace Injectors

High-precision volumes

Analytical Software

Chromatography data

Analytical
Integrity

LOD/LOQ analytical limits determinations
Agilent systems with FLD, PDA & UV-Vis
Chemical, Protein, & Antibody Quantitation
Complete deformulation and ingredient testing
Detailed accuracy, recovery, & selectivity protocols
Data Validated
High-Sensitivity

"Fluorescence detectors are highly sensitive, best suited for cases where analytical compounds are inherently fluorescent or have been selectively modified to be so."

Molecular Purity Analytics

HPLC Specialized Tiers

Scale of Method Execution

Quantitation Run

Standard Separation

Standard molecule quantitation
Reporting of major peaks
UV-Vis detection parameters
Chromatogram delivery
Diagnostic Standard

Master Deformulation

Full Ingredient Profile

Complete product deformulation
Impurity identification
CAS Number validation
Formula quantitation & cost analysis

Custom Validation

Method Development

LOD/LOQ validation runs
Precision & Accuracy metrics
Linearity & Range definition
Regulatory-compliant dossier

Analytes
Decoded

Download our technical handbook to explore the analytical capacity of HPLC deformulations, method developments, and trace fluorescence assays.

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