Custom Gene
Cloning Services
Precision vector construction and recombinant DNA engineering to accelerate your therapeutic research and protein discovery.
The Foundation of
Molecular Engineering
Gene cloning is the process of isolating and creating multiple copies of a specific gene or DNA segment. It serves as the cornerstone of biotechnology, enabling the study of gene function, production of therapeutic proteins, and engineering of organisms.
Ponnaiya CAG provides end-to-end solutions, from sequence-verified vector construction to large-scale library generation for therapeutic and industrial applications.
Vector Design
Customized regulatory elements and tags.
PCR Subcloning
High-efficiency directional insertion.
Protein Ready
Expression-optimized constructs.
Full Accuracy
100% Sequence-verified results.
Cloning & Custom Vector Services
Custom Vector Construction
De novo design and synthesis of specialized vectors tailored for your specific expression systems.
PCR Subcloning
Transferring DNA fragments into high-expression vectors using restriction-based or seamless cloning.
Library Construction
Generation of high-quality genomic or cDNA libraries for functional screening and discovery.
Vector Engineering
Modification of existing vectors to enhance stability, expression yield, or modularity.
Sequence Verification
Rigorous verification of the final construct using Sanger sequencing to ensure 100% accuracy.
Therapeutic Cloning Support
Foundational services for monoclonal antibody research and recombinant vaccine development.
Project Execution Pipeline
Insert Preparation
PCR or synthetic fragment
Vector Linearization
Restriction or PCR opening
Assembly / Ligation
Isothermal or T4 ligation
Transformation
High-efficiency competence
Verification
Colony PCR & Sequencing
QA Guarantee:All cloned inserts are sequence-verified over the entire reading frame using Sanger sequencing to ensure mutation-free delivery of your genetic blueprint.
Applications of Gene Cloning
Driving innovation across biotechnology and therapeutic research.
Recombinant Proteins
Transgenic Development
Functional Genomics
Therapeutic Research
Vaccine Development
MAb Discovery
Why Ponnaiya CAG?
"We deliver standardized, expression-ready vectors that serve as reliable templates for diagnostics, vaccines, and monoclonal antibody development."
Cloning Packages
Tailored genetic engineering solutions
Basic Cloning
Standard PCR Subcloning
Vector Pro
Custom Construction
Library Scale
Complex Constructions
Explore Vector
Built Specs
Download our technical brochure for details on available vectors, promoter options, and cloning methodologies.