Molecular Lab
From Detection to Discovery — Quantify Life at Molecular Resolution

Real-Time PCR
(qPCR) Analysis

Precision-driven DNA & RNA quantification for diagnostics, research, and molecular innovation.

What is Real-Time PCR?

Real-Time PCR (qPCR) is an advanced molecular technique that amplifies and quantifies DNA or RNA simultaneously during each cycle, enabling accurate, real-time measurement of genetic material.

Unlike conventional PCR, results are captured dynamically using fluorescence signals, delivering high sensitivity, precision, and speed.

Real-time detection

No post-processing required, instant visibility.

High sensitivity

Detection of even the smallest genetic changes.

Quantitative results

Absolute & relative quantification models.

High-throughput

Scalable for large studies and rapid diagnostics.

Our qPCR Service Capabilities

01

Pathogen Detection & Diagnostics

Rapid identification and quantification of bacteria, viruses, and fungi across clinical, food, and environmental samples.

Infectious disease detection
Microbial load analysis
Environmental monitoring
02

Gene Expression Analysis

Quantify gene activity with high precision using cDNA-based amplification.

RT-qPCR workflows
Differential gene expression
Functional genomics
03

Genetic Testing & Disease Profiling

Advanced molecular diagnostics for identifying mutations and disease risks.

Cancer biomarker analysis
Mutation detection
Clinical diagnostics
04

DNA & RNA Quantification

Accurate measurement of nucleic acid concentration in samples.

Absolute & relative quantification
Copy number analysis
Template validation
05

Genotyping & SNP Analysis

Fast, high-throughput genetic variation analysis.

SNP detection
Allelic discrimination
Population genetics
06

Custom Assay Development

Tailored qPCR assay design for research and industry-specific needs.

Primer & probe design
Assay optimization
Multiplex qPCR

Our qPCR Workflow

1

Sample Preparation

(DNA/RNA extraction)

2

Reverse Transcription

(for RNA → cDNA)

3

Reaction Setup

(primers, probes, reagents)

4

Amplification & Detection

(real-time fluorescence)

5

Data Analysis

(Ct values, quantification)

Micro Note:Fluorescence intensity correlates directly with nucleic acid concentration during amplification cycles.

Where qPCR Powers Innovation

Biomedical Research

Drug Discovery & Validation

Infectious Disease Diagnostics

Agricultural Genomics

Environmental Monitoring

Food Safety Testing

Why Ponnaiya CAG?

State-of-the-art molecular biology lab
Expert scientists & bioinformatics integration
Fast turnaround with scalable pipelines
High-accuracy quantitative reporting
End-to-end support (from experiment to insight)
99.9%
Accuracy & Reproducibility

"Our commitment to precision ensures that your molecular research moves from detection to discovery with absolute confidence."

Service Packages

Tailored qPCR solutions for every research stage

Basic Plan

Expression Analysis

RNA extraction QC
RT-qPCR setup
Gene expression report
Most Popular

Pro Plan

Advanced Molecular Insights

All Basic features
Differential expression analysis
Disease-specific markers / trait-specific genes

Premium Plan

Deep Genomic Profiling

All Pro features
SNP / mutation profiling
Custom assay + advanced interpretation

Explore Full Capabilities

Download our technical brochure for detailed specifications, sample requirements, and multi-plex assay options.

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