Amino Acid
Analysis (AAA)
Industry-standard IEC and high-performance HPLC platforms for the precise quantification of free and bound amino acids across biological and nutritional matrices.
Precision
Compositional Mapping
Accurate amino acid analysis (AAA) is a critical tool for recombinant protein characterization, nutritional assessment, and pharmaceutical purity testing. Precise quantification is essential whether AAs exist in a free state or are bound in complex linear chains.
We provide granular data on the full spectrum of natural amino acids using industry-standard Ion-Exchange Chromatography (IEC) paired with post-column Ninhydrin derivatization for exceptional quantitative precision.
IEC Industry Std
IEC + Ninhydrin for elite quantitative accuracy.
Full Spectrum
Quantification of all 20+ natural amino acids.
Bound vs Free
Analysis of both peptides and free substrates.
MS Validation
HPLC-MS for unknown fragment identification.
Analytical AAA Capabilities
Full Spectrum Quantification
Precise measurement of all 20+ natural amino acids, including acidic, basic, and sulfur-containing groups.
IEC & Ninhydrin Detection
Industry-standard Ion-Exchange Chromatography paired with post-column Ninhydrin derivatization.
HPLC Profiling
Advanced profiling using HPLC with UV, PDA, and High-Sensitivity Fluorescence (FLD) detectors.
Hydrolysis & Partitioning
Standardized acid/base hydrolysis to release bound amino acids from complex peptide chains.
Nutritional & Pharma QC
Granular data generation for nutritional labeling, animal feed, and pharmaceutical purity testing.
LC-MS Analysis
Coupling HPLC with Mass Spectrometry for structural validation and unknown fragment identification.
Technical AAA Pipeline
Sample Prep / Login
Standardized intake & login
Acid/Base Hydrolysis
Releasing bound amino acids
IEC Separation
Ion-exchange chromatography
Derivatization
Post-column Ninhydrin run
Quantification
Photometric & HPLC scaling
Post-Column Derivatization ExcellenceIndustry Prep
"We utilize the industry-standard method of Ion-Exchange Chromatography (IEC) paired with post-column Ninhydrin derivatization. This method is preferred for its exceptional qualitative and quantitative precision, providing the granular data needed for nutritional labeling and pharmaceutical purity testing."
Full Spectrum Analysis
Quantifying the 20+ building blocks of molecular biological systems.
Acidic / Amides
Aspartic acid, Glutamic acid, Asparagine, Glutamine
Basic Groups
Lysine, Histidine, Arginine
Aliphatic
Alanine, Glycine, Valine, Leucine, Isoleucine, Proline
Sulfur / Aromatic
Cysteine, Methionine, Tyrosine, Phenylalanine, Tryptophan
Precision
Compositional Certainty
"From determining relative amino acid composition to identifying free amino acids in biological samples, our services provide the granular data needed for pharmaceutical purity and research."
AAA Profiling Packages
Architecture of Purity
Free Profile
Non-Hydrolyzed AA
Total Composition
Bound + Free AA
Pharma/MS Tier
High-Sensitivity Run
Purity
Without Margin
Download our technical handbook to explore IEC-Ninhydrin methodologies, HPLC detector options, and standard AAA groups.