Key Applications
Agricultural Genomics
Improve crop yield, disease resistance, and environmental stress tolerance by understanding the full genetic blueprint of elite cultivars.
Biodiversity & Conservation
Establish high-quality reference genomes for endangered species to support conservation genetics and evolutionary studies.
Microbiology & Microbiomics
Fully resolve complex microbial genomes, plasmids, and structural variants without the need for a prior reference.
Pharmacognosy & Drug Discovery
Discover novel biosynthetic gene clusters in medicinal plants and marine organisms for pharmaceutical applications.
The Technology Trinity
One platform is never enough for a perfect assembly. We weave together long-reads and spatial mapping to resolve the unresolvable.
PacBio HiFi (CCS)
Combining the accuracy of short-read sequencing with the length of long-reads for near-perfect contig assembly.
Oxford Nanopore (ULR)
Ultra-Long Reads (100kb+) to span the most complex repetitive regions and structural variations.
Hi-C Scaffolding
Capturing 3D chromatin interactions to anchor contigs into chromosome-level pseudomolecules.
Assembly
Gold Standards
Building a reference-grade genome requires more than just raw data. Our computational pipeline utilizing Hifiasm, Canu, and Salsa2 ensures that N50 metrics and BUSCO scores exceed international consortium standards.
- Haplotype-Aware Phase Assembly
- Gap-Free Centromere & Telomere Resolution
- High-Confidence Gene Scaffolding
- Automated Assembly Polishing with Illumina
Conquering Polyploidy & Repeats
Many plant genomes are polyploid (multiple chromosome sets), making assembly extremely difficult. We utilize Oxford Nanopore Ultra-Long reads to span multi-megabase repeats and PacBio HiFi to resolve highly similar haplotypes.
Heterozygosity Handling
Advanced algorithms to separate paternal and maternal alleles in diploid organisms.
Chromosomal Scaffolding
Utilizing Hi-C to map long-range connections and anchor contigs into full chromosomes.
End-to-End Pipeline
HMW DNA Extraction
Specialized protocols for extracting High Molecular Weight DNA from complex plant/animal tissues.
Multi-Platform Sequencing
Combining PacBio HiFi, Oxford Nanopore Ultra-Long, and Illumina for comprehensive coverage.
Hi-C Library Prep
Capturing 3D chromatin conformation data to anchor contigs into full chromosomes.
De Novo Assembly
Advanced algorithmic assembly (Hifiasm) and polishing for error-free contig generation.
Annotation & Delivery
Gene prediction, repeat masking, functional annotation, and secure data handover.
Supported Sample Types & Requirements
* Note: Flash-freezing in liquid nitrogen is mandatory for plant/animal tissue samples to preserve HMW DNA integrity.
Client Deliverables
Technical FAQ
Build Your Species' Blueprint.
Join the elite group of researchers publishing chromosome-level reference genomes with our Technology Trinity.