The Technology Trinity
One platform is never enough for a perfect assembly. We weave together long-reads and spatial mapping to resolve the unresolvable.
PacBio HiFi (CCS)
Combining the accuracy of short-read sequencing with the length of long-reads for near-perfect contig assembly.
Oxford Nanopore (ULR)
Ultra-Long Reads (100kb+) to span the most complex repetitive regions and structural variations.
Hi-C Scaffolding
Capturing 3D chromatin interactions to anchor contigs into chromosome-level pseudomolecules.
Assembly
Gold Standards
Building a reference-grade genome requires more than just raw data. Our computational pipeline utilizing Hifiasm, Canu, and Salsa2 ensures that N50 metrics and BUSCO scores exceed international consortium standards.
- Haplotype-Aware Phase Assembly
- Gap-Free Centromere & Telomere Resolution
- High-Confidence Gene Scaffolding
- Automated Assembly Polishing with Illumina
Conquering Polyploidy & Repeats
Many plant genomes are polyploid (multiple chromosome sets), making assembly extremely difficult. We utilize Oxford Nanopore Ultra-Long reads to span multi-megabase repeats and PacBio HiFi to resolve highly similar haplotypes.
Heterozygosity Handling
Advanced algorithms to separate paternal and maternal alleles in diploid organisms.
Chromosomal Scaffolding
Utilizing Hi-C to map long-range connections and anchor contigs into full chromosomes.
End-to-End Pipeline
Genome Survey
Illumina depth + K-mer analysis
Long-Read Assembly
Primary contig generation
Hi-C Scaffolding
Chromosome anchoring
Gene Annotation
Ab-initio & Homology based
Technical FAQ
Build Your Species' Blueprint.
Join the elite group of researchers publishing chromosome-level reference genomes with our Technology Trinity.